#Light scattering intensity equation Patch

A correlator is basically a signal comparator.

Intensity fluctuations seen through time are therefore slower for large particles.The mass of the particles has almost no influence on the rate of particle diffusion, and may safely be ignored.

Particles with a large physical dimension (radius) diffuse more slowly through a solvent, while small particles diffuse more quickly.

#Light scattering intensity equation Patch

The bright blobs of light in the speckle pattern are where the light scattered from the particles arrives with the same phase and interfere constructively to form a bright patch (B). The dark spaces are where the phase additions of the scattered light are mutually destructive and cancel each other out (A). A classical speckle pattern would be seen The speckle pattern will be stationary both in speckle size and position because the whole system is stationary. How do these fluctuations in the intensity of scattered light arise?Imagine if a cuvette, containing particles which are stationary, is illuminated by a laser and a frosted glass screen is used to view the sample cell. This is done by measuring the rate at which the intensity of the scattered light fluctuates when detected using a suitable optical arrangement. In dynamic light scattering, the speed at which the particles are diffusing due to Brownian motion is measured.

Includes hydration and shape effects.Other Common Radius DefinitionsRadius of Rotation (RR): The radius of a sphere defined by rotating the protein about the center of mass.Mass Radius (RM): The radius of a hard sphere of the same mass and density of the protein.Radius of Gyration (Rg): The mass weighted average distance from the center of mass to every atom in the protein Hydrodynamic Radius (RH): The radius of a hard sphere that diffuses at the same rate as the protein.